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51.
Study of interaction between DNA and intercalator at molecular level is important to understand the mechanisms of DNA replication and repair. A micro-fabricated local heating thermodevice was adapted to perform denaturation experiments of DNA with fluorescent intercalator on millisecond time scale. Response time of complete unzipping of double stranded DNA, 16 μm in length, was measured to be around 5 min by commercial thermocycler. Response time of quenching of double stranded DNA with fluorescent intercalator SYBR Green was measured to be 10 ms. Thus, quenching properties owing to strand unzipping and denaturation at base pair level were distinguished. This method has provided easy access to measure this parameter and may be a powerful methodology in analyzing biomolecules on millisecond time scale. 相似文献
52.
53.
Satoko Akashi Kazuyoshi Noguchi Reiko Yuji Uno Tagami Kazuo Hirayama Koichi Kato HaHyung Kim Kazuhiro Tokioka Ichio Shimada Yoji Arata 《Journal of the American Society for Mass Spectrometry》1996,7(8):707-721
The amino acid sequences of mouse monoclonal antibodies have been characterized completely by mass spectrometry. Antibodies used in the present study were derived from mouse switch variant cell lines that produce four kinds of immunoglobulin Gs (IgGs). The amino acid sequences of these antibodies had not been estimated from the corresponding DNA sequence, so the sequences of IgGs derived from other strains were used as references in this study. Intra- and interchain disulfide bonds of the IgGs were reduced and carboxymethylated and the products were subjected to proteolytic digestion. The existence of N-linked oligosaccharides also was taken into account. The capabilities and limitations of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and capillary liquid chromatography-electrospray ionization mass spectrometry are discussed in the structural characterization of the antibodies. Based on our results, allotypes of the antibodies examined are discussed. This study shows that amino acid sequences of proteins, such as IgG, can be investigated without information about the corresponding DNA sequence if appropriate reference sequences derived from other strains can be used. 相似文献
54.
Hiromi Matsuhashi Daishi Sato Kazushi Arata 《Reaction Kinetics and Catalysis Letters》2004,81(1):183-188
The acidity of sulfated alumina catalysts was studied by analyzing DTG and heat of Ar adsorption together with the isomerization of pentane. The initial acid sites were Brönsted-type, and converted into Lewis-type upon increasing the pretreatment temperature. The heat of Ar adsorption of the most active sulfated alumina was 18.9 kJ mol–1, this value being a little smaller than that of sulfated zirconia (23.6 kJ mol–1).This revised version was published online in December 2005 with corrections to the Cover Date. 相似文献
55.
The first synthesis of a stereoisomeric mixture of hormone α1, the mating hormone of Phytophthora, was achieved, and the synthetic mixture was confirmed to be hormonally active. 相似文献
56.
The relative acid strength and acid amount of solid acids has been determined from the adsorption and desorption of small molecules, such as argon. The order of activation energy for desorption of Ar from a solid acid, determined using temperature-programmed desorption (TPD), is sulfated zirconia > Cs2.5H0.5PW12O40 > proton-type zeolites > silica–alumina. The adsorption isotherms were analyzed using Langmuir and Henry equations. The Henry-type adsorption isotherms were also analyzed using the theory of Cremer and Flügge. The heat of Ar adsorption was 22 kJ mol−1 for sulfated zirconia and ca. 17 kJ mol−1 for mordenite, ZSM-5, and beta-zeolite. Molybdenum oxides reduced at 623 and 773 K exhibited a large heat of adsorption (19.3 and 19.7 kJ mol−1, respectively), and these materials are classified as superacids. W-Nb mixed-oxides and tungstated tin oxide (calcined at 1373 K), which are newly developed solid acids, had a heat of adsorption of 18.1 and 16.9 kJ mol−1, respectively. The type of acid site could be distinguished by comparing the heat of adsorption of Ar and N2. Our data indicate that Ar is useful for the characterization of solid acids. 相似文献
57.
Gonzalez V Wilson T Kurihara I Imai A Thomas JA Otsuki J 《Chemical communications (Cambridge, England)》2008,(16):1868-1870
A dinuclear ruthenium(II) complex groove binds to DNA and this interaction results in distinctive color changes that are dependent on both DNA sequence and structure. 相似文献
58.
Real-time observation of biomolecular behavior focusing on high speed temperature response is an essential endeavor for further biological study at the molecular level. This is because most of the important biological functions at the molecular level happen at the sub-second time scale. We used our own on-chip microheaters and microcontainers to observe the denaturation dynamics of fluorescent proteins at the millisecond time scale. The microheater controls the temperature in 1 ms under the microscope. Fluorescent proteins were contained in 28 fL PDMS microcontainers to prevent them from diffusing into the solution. The proteins were denatured by high temperatures and observed by a high speed CCD camera with 5 ms per frame. Hence, denaturation speeds of red fluorescent proteins (rDsRed and rHcRed) were measured to be 5-10 ms. Green fluorescent proteins (rAcGFP and rGFPuv) denatured with bi-exponential decay. rAcGFP denatured with time constants of 5 ms and 75 ms while rGFPuv denatured with 10 ms and 130 ms. This may be the reverse process of a two step renaturation of GFP observed in a previous report. This micro-thermodevice is applicable to other biomaterials such as nucleic acids or other proteins. It does not require any chemical treatment nor mutation to the biomaterial itself. Therefore, the methodology using this general purpose device gives access to biomolecular studies in short time scales and acts as a powerful tool in molecular biology. 相似文献
59.
Yamaguchi S Matsumoto S Ishizuka K Iko Y Tabata KV Arata HF Fujita H Noji H Hamachi I 《Chemistry (Weinheim an der Bergstrasse, Germany)》2008,14(6):1891-1896
The artificial regulation of protein functions is essential for the realization of protein-based soft devices, because of their unique functions conducted within a nano-sized molecular space. We report that self-assembled nanomeshes comprising heat-responsive supramolecular hydrogel fibers can control the rotary motion of an enzyme-based biomotor (F(1)-ATPase) in an on/off manner at the single-molecule level. Direct observation of the interaction of the supramolecular fibers with a microbead unit tethered to the F(1)-ATPase and the clear threshold in the size of the bead required to stop ATPase rotation indicates that the bead was physically blocked so as to stop the rotary motion of ATPase. The temperature-induced formation and collapse of the supramolecular nanomesh can produce or destroy, respectively, the physical obstacle for ATPase so as to control the ATPase motion in an off/on manner. Furthermore, this switching of the F(1)-ATPase motion could be spatially restricted by using a microheating device. The integration of biomolecules and hard materials, interfaced with intelligent soft materials such as supramolecular hydrogels, is promising for the development of novel semi-synthetic nano-biodevices. 相似文献
60.
Kawabata Masako Motoishi Shoji Ohta Akio Motomura Arata Saeki Hideya Tsukada Kazuaki Hashimoto Shintaro Iwamoto Nobuyuki Nagai Yasuki Hashimoto Kazuyuki 《Journal of Radioanalytical and Nuclear Chemistry》2021,330(3):913-922
Journal of Radioanalytical and Nuclear Chemistry - Both 64Cu and 67Cu are promising radionuclides in nuclear medicine. Production yields of these radionuclides were quantified by irradiating... 相似文献